Genetics Graduate Program: Faculty
L. Patrick Hart 

Professor of Plant Pathology; 

Ph.D., 1978, University of California, Riverside.

Email
hart1@pilot.msu.edu
Phone: (517) 353-9428
Lab: 353-2040
Home Department: Plant Pathology 
Home Page:
http://www.plantpathology.msu.edu/
profiles/hart.htm

Early recognition of fungal pathogens by the host plant shortly after inoculation is an important component of the plant defensive response. Unfortunately, no host receptors have been identified that can clearly distinguish between compatible and incompatible genera, species or races of pathogenic fungi. The host­specific toxin victorin appears to interact with a host protein, possibly a receptor, located on the plasma membrane. The sensitivity or insensitivity of specific cultivars of oats to victorin is important because there is a direct correlation between sensitivity to victorin and resistance to the oat rust pathogen Puccinia coronata. Classical genetic studies indicate the role of a single Mendelian gene involved in the response to victorin. Research in my laboratory is directed toward characterization of the victorin receptor and clarification of its role in oat resistance to P. coronata by using immunological techniques.

In support of my immunological approach to answering some basic questions of host­pathogen interactions, I am also developing protocols to produce a wider range of antibody idiotypes and anti­idiotypes by gene cloning and random recombination of light and heavy chain cDNA libraries. This approach has advantages over traditional polyclonal and monoclonal techniques.

SELECTED PUBLICATIONS:

Qiaoling Jin, Wenqi Hu, Ian Brown, Gayle McGhee, Patrick Hart, Alan L. Jones, and Sheng Yang He. 2001. Visualization of secreted Hrp and Avr proteins along the Hrp pilus during type lll secretion in Erwinnia amylovora and Pseudomonas syringae. Molecular Microbiology 40: 1129-1139.

Wenqi Hu, Jing Yuan, Qiao-Ling Jin, Patrick Hart, and Sheng Yang He. 2001. Immunogold Labeling of Hrp Pili of Pseudomonas syringae pv. tomato in Minimal Medium and in planta. Mol. Plant-Microbe Interact. Vol. 14, No. 2, 2001, pp. 234B241.

Kaitany, R., L. P. Hart, and G. Safir. 200_. Race composition of Phytophthora sojae in Michigan. Plant Disease (in press)

M. G. Lee, Q. P. Yuan, L. P. Hart, and J. J. Pestka. 2000. Enzyme-Linked immunosorbent assays of zearalenone using polyclonal, monoclonal and recombinant antibody. Eds. M. W. Truckess and A. E. Pohland. Methods in Molecular Biology, Vol. 57: Mycotoxin Protocols, 159-170. Humana Press.

Yuan, Q., W. Hu, J. J. Pestka, S. He, and L. P. Hart. 2000. Expression of a functional anti-zearalenone antibody in transgenic Arabidopsis plants. Appl. Environ. Microbiol. 66:3499-3505.

Akimitsu K., J. Walton and L.P. Hart (1993) Density gradient studies of victorin binding proteins in oats. Plant Physiology 103:67­72.

Akimitsu K., J. Walton and L.P. Hart (1993) Immunological evidence for a cell­surface receptor of victorin using anti­victorin anti­idiotypic polyclonal antibodies. Molecular Plant­Microbe Interactions 6:429­433.

Akimitsu K., L.P. Hart, J. Walton and R. Hollingsworth (1992) Covalent binding sites of victorin in oat leaf tissues detected by anti­victorin polyclonal antibody. Plant Physiology 98:121­126.

Other Publications
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